African Journal of Biotechnology
WEBMay 10, 2017 · The leaves were washed with tap water, surface dried at room temperature, and freeze dried to make leaf powder. Freeze dried leaves were ground into powder using a ceramic ball mill (SWBM117, Samwoo Engineering, Seoul, Korea) and strained using a series of US standard testing sieves (60 mesh) stacked in the portable sieve shaker (M .